Plasmid
Part:BBa_K5353065:Design
Designed by: Daoming MA Group: iGEM24_UM-Macau (2024-10-01)
1108 LentiV-Lamp2b/hTRPC1/HA: Neo
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal XbaI site found at 843
Illegal XbaI site found at 1593
Illegal PstI site found at 715
Illegal PstI site found at 1101
Illegal PstI site found at 1465
Illegal PstI site found at 1851 - 12INCOMPATIBLE WITH RFC[12]Illegal PstI site found at 715
Illegal PstI site found at 1101
Illegal PstI site found at 1465
Illegal PstI site found at 1851 - 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 4179
Illegal BamHI site found at 2574
Illegal XhoI site found at 3869 - 23INCOMPATIBLE WITH RFC[23]Illegal XbaI site found at 843
Illegal XbaI site found at 1593
Illegal PstI site found at 715
Illegal PstI site found at 1101
Illegal PstI site found at 1465
Illegal PstI site found at 1851 - 25INCOMPATIBLE WITH RFC[25]Illegal XbaI site found at 843
Illegal XbaI site found at 1593
Illegal PstI site found at 715
Illegal PstI site found at 1101
Illegal PstI site found at 1465
Illegal PstI site found at 1851 - 1000COMPATIBLE WITH RFC[1000]
Design Notes
Used the CMV promoter, a strong protomer to drive high level Lamp2b-hTRPC1-HA gene expression in HEK293T cell.
Source
The sequence of the parts used in this plasmid were retrieved from GenBank.