Plasmid

Part:BBa_K5353065:Design

Designed by: Daoming MA   Group: iGEM24_UM-Macau   (2024-10-01)


1108 LentiV-Lamp2b/hTRPC1/HA: Neo


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal XbaI site found at 843
    Illegal XbaI site found at 1593
    Illegal PstI site found at 715
    Illegal PstI site found at 1101
    Illegal PstI site found at 1465
    Illegal PstI site found at 1851
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 715
    Illegal PstI site found at 1101
    Illegal PstI site found at 1465
    Illegal PstI site found at 1851
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 4179
    Illegal BamHI site found at 2574
    Illegal XhoI site found at 3869
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal XbaI site found at 843
    Illegal XbaI site found at 1593
    Illegal PstI site found at 715
    Illegal PstI site found at 1101
    Illegal PstI site found at 1465
    Illegal PstI site found at 1851
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal XbaI site found at 843
    Illegal XbaI site found at 1593
    Illegal PstI site found at 715
    Illegal PstI site found at 1101
    Illegal PstI site found at 1465
    Illegal PstI site found at 1851
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Used the CMV promoter, a strong protomer to drive high level Lamp2b-hTRPC1-HA gene expression in HEK293T cell.


Source

The sequence of the parts used in this plasmid were retrieved from GenBank.

References